Abstract

Continuous low-level supply or in situ generation of hydrogen peroxide (H2O2) is essential for
the stability of unspecific peroxygenases, which are deemed ideal biocatalysts for the
selective activation of C–H bonds. To envisage potential large scale applications of combined
catalytic systems the reactions need to be simple, efficient and produce minimal by-products.
We show that gold-palladium nanoparticles supported on TiO2 or carbon have sufficient
activity at ambient temperature and pressure to generate H2O2 from H2 and O2 and supply
the oxidant to the engineered unspecific heme-thiolate peroxygenase PaDa-I. This tandem
catalyst combination facilitates efficient oxidation of a range of C-H bonds to hydroxylated
products in one reaction vessel with only water as a by-product under conditions that could
be easily scaled.

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