Journal article

Brief Report: HIV-1 Infection Impairs CD16 and CD35 Mediated Opsonophagocytosis of Mycobacterium tuberculosis by Human Neutrophils


Research Areas

Currently no objects available


Publication Details

Author list: Lowe D., Bangani N., Nakiwala J., Martineau A., Wilkinson R., Wilkinson K.

Publication year: 2016

Journal: JOURNAL OF ACQUIRED IMMUNE DEFICIENCY SYNDROMES AND HUMAN RETROVIROLOGY

Journal name: Journal of Acquired Immune Deficiency Syndromes

Volume number: 73

Issue number: 3

Start page: 263

End page: 267

Total number of pages: 5

ISSN: 1077-9450

URL: https://api.elsevier.com/content/abstract/scopus_id/84973343112


Abstract

Using a flow cytometric assay, we investigated neutrophil-Mycobacterium tuberculosis opsonophagocytosis and the impact of HIV-1-infected serum on this process. The mean (±SD) percentage of neutrophils internalizing bacilli after 30 minutes incubation was significantly reduced by pretreatment with anti-CD16 (18.2% ± 8.1%, P < 0.001) or anti-CD35 antibody (23.2% ± 10.6%, P < 0.05) versus anti-CD4 controls (29.9% ± 8.1%). Blocking CD88 or CD11a did not affect internalization. Using heat-inactivated serum, maximal internalization was lower using HIV-1-infected serum versus HIV-1-uninfected. Using non-heat-inactivated serum, internalization decreased more rapidly with sequential dilutions of HIV-1-infected versus HIV-1-uninfected serum. CD16 and CD35 are important for neutrophil internalization of M. tuberculosis, whereas HIV-1 infection adversely affects opsonophagocytosis.


Projects

Currently no objects available


Keywords

HIV, Tuberculosis


Last updated on 2017-02-05 at 15:25