Journal article

Streptomyces swartbergensis sp. nov., a novel tyrosinase and antibiotic producing actinobacterium


Research Areas

Currently no objects available


Publication Details

Author list: le Roes-Hill M., Prins A., Meyers P.

Publisher: Springer Verlag (Germany)

Publication year: 2018

Journal: Antonie van Leeuwenhoek

Journal name: Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology

Volume number: 111

Issue number: 4

Start page: 589

End page: 600

Total number of pages: 12

ISSN: 0003-6072

eISSN: 1572-9699

URL: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85033490905∨igin=inward


Abstract

As part of an antibiotic screening program, an actinobacterium, strain HMC13T, was isolated from soil collected from the banks of the Gamka River, Western Cape Province, South Africa. The isolate was found to produce branched mycelia that differentiated into spiral spore chains with spiny spores. 16S rRNA gene sequence analysis showed the strain to be closely related to Streptomyces caelestis NRRL 2418T (99.72%) and Streptomyces azureus NBRC 12744T(99.51%). Chemotaxonomic analyses confirmed the classification of the strain as a member of the genus Streptomyces: LL-DAP in the peptidoglycan, no diagnostic sugars in the whole cell sugar pattern, dominant menaquinones including MK9(H8), MK9(H6), and the polar lipids detected included phosphatidylethanolamine. The fatty acid profile revealed the presence of mostly branched, saturated fatty acids: iso-C15:0 (14.4%), anteiso-C15:0 (21.1%), iso-C16:0(16.8%), C16:1ω7c/2-OH iso-C15:0 (5.8%), C16:0 (6.2%), iso-C17:1ω9c (5.8%), iso-C17:0 (5.9%), and anteiso-C17:0 (9.6%). Strain HMC13T is a tyrosinase producer and exhibits very strong antibiosis against Mycobacterium aurum A+ and Staphylococcus aureus subsp. aureus ATCC 33591 (methicillin resistant), while only weak activity was observed against Bacillus cereus ATCC 10876, Enterococcus faecium VanA (vancomycin resistant), Enterococcus faecalis ATCC 51299 (vancomycin resistant) and Candida tropicalis ATCC 750T. Strain HMC13T (= LMG 28849T = NRRL B-65294T) is proposed as the type strain of a new species, to be named Streptomyces swartbergensis sp. nov.


Projects

Currently no objects available


Keywords

antimicrobial


Last updated on 2019-18-04 at 10:01